HSP70 Monoclonal Antibody (3A3)
Invitrogen
catalog: MA3-006

total number of products: 7

mouse monoclonal (3A3)
reactivity: Japanese rice fish, sea monkeys, common limpet, red rock crab, Eyprepocnemis plorans, giant tiger prawn, eastern oyster, lobsters, Chlamydomonas reinhardtii, roach minnow, Artemia franciscana, Saccharomycetales, holothurians, Pacific oyster, crucian carp, Marenzelleria viridis, Asian green mussel, Meganyctiphanes norvegica, snow fleas, human, mouse, rat, dogs, pigs , fruit fly , roundworm , rhesus macaque
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments
citations: 98

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) (Product# MA3-006) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-006) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Western blot analysis of Heat Shock Protein 70 (Hsp70) was performed by loading 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody (Product # MA3-006) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
quantity: 50 ug
price: US 538.00
to the supplier

HSP70 Monoclonal Antibody (C92F3A-5), APC
Invitrogen
catalog: MA5-45090

total number of products: 7

mouse monoclonal (C92F3A-5)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, domestic rabbit, roundworm
conjugate: APC
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 200 ug
price: US 470.00
to the supplier

HSP70 Monoclonal Antibody (C92F3A-5), FITC
Invitrogen
catalog: MA5-45091

total number of products: 7

mouse monoclonal (C92F3A-5)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, domestic rabbit, roundworm
conjugate: FITC
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 200 ug
price: US 454.00
to the supplier