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product
- mouse monoclonal (RL90)
reactivity: Chinese hamster, African green monkey, human, mouse, rat, pig, Rhesus monkey
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
citations: 68
Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
quantity: 100µL
price: 381 USD
to the supplier - mouse monoclonal (RL77)
reactivity: African green monkey, Chinese hamster, human, mouse, rat, dog, pig, common platanna, Rhesus monkey
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments
citations: 37 - PDI Monoclonal Antibody (RL90), Alexa Fluor 488
Invitrogen
catalog: MA3-019-A488
total number of products: 5mouse monoclonal (RL90)
reactivity: Chinese hamster, human, mouse, rat, pig
conjugate: AF488
application: immunocytochemistry
citations: 3
gene information - pig P4HB
- synonym:protein disulfide-isomerase; prolyl 4-hydroxylase beta polypeptide
- description:prolyl 4-hydroxylase subunit beta
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product type
- P4HB antibody
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