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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 3
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human Jurkat T cell leukemia cell lysate (lanes 3 and 6): - 1-3. Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:400).4-6. Anti-VPAC1 (VIPR1) (extracellular) Antibody, preincubated with VPAC1/VIPR1 (extracellular) Blocking Peptide (#BLP-VR001).
Expression of VPAC1 in rat amygdala - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:100). A. VPAC1 staining (red) is apparent in basolateral amygdala neurons (horizontal arrow). B. Cell nuclei in the same section are stained with DAPI (Blue). C. Merge of the two images.
Cell surface detection of VPAC1 in live intact Jurkat (human T cell leukemia cells) cell line: - ___Cells + goat-anti-rabbit-DyLight-488.___Cells +Anti-VPAC1 (VIPR1) (extracellular) Antibody(#AVR-001) (1:20) + goat-anti-rabbit-DyLight-488.
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
citations: 1 
Cell surface detection of VPAC1 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of VPAC1 in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Black line: Cells + rabbit IgG isotype control-FITC. Black line: Cells + Anti-VPAC1 (VIPR1) (extracellular)-FITC Antibody (#AVR-001-F), (2.5 µg).
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to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal (PD00-96)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: HT-29 cell lysate (20 µg/Lane) Lane 5: Caco-2 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: MDA-MB-231 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: LNCaP cell lysate (20 µg/Lane) Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-VPAC1 antibody (HA721209) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721209) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of VPAC1 on different lysates with Rabbit anti-VPAC1 antibody (HA721209) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: COS-1 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: L6 cell lysate (20 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Mouse lung tissue lysate (40 µg/Lane) Lane 8: Mouse small intestine tissue lysate (40 µg/Lane) Lane 9: Mouse colon tissue lysate (40 µg/Lane) Lane 10: Rat lung tissue lysate (40 µg/Lane) Lane 11: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721209) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - mouse Vipr1
- synonym:AV071699; VIP-R1; VPAC1
- description:vasoactive intestinal peptide receptor 1
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product type
- Vipr1 antibody
- Vipr1 gene knockdown
- Vipr1 cDNA
- Vipr1 other
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