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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 10
Western blot analysis of Human PC-3 prostate cancer cell lysate: - 1. Anti-TRPV6 Antibody (#ACC-036), (1:200).2. Anti-TRPV6 Antibody, preincubated with TRPV6 Blocking Peptide (#BLP-CC036).
Expression of TRPV6 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV6 Antibody (#ACC-036), (1:100). A. TRPV6 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV6 and Parvalbumin demonstrates colocalization (arrows).
Western blot analysisof PC-3 cell lysate: - 1.Anti-TRPV6Antibody (#ACC-036) (1:200).2. Anti-TRPV6 Antibody preincubated with the negative control antigen.
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of TRPV6 on different lysates with Rabbit anti-TRPV6 antibody (HA500333) at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: SK-Br-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 42 kDa (unglycosylated monomeric form of TRPV6) Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500333) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ICC staining of TRPV6 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500333, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of TRPV6 in HT-29 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500333, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
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gene information - mouse Trpv6
- synonym:CAT; CaT1; Cac; Ecac2; Otrpc3
- description:transient receptor potential cation channel, subfamily V, member 6
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product type
- Trpv6 antibody
- Trpv6 gene knockdown
- Trpv6 cDNA
- Trpv6 other
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