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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1 - mouse monoclonal (G-3)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 1 - mouse monoclonal (G-3)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (G-3)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (PSH18-09)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of TPP1 on different lysates with Rabbit anti-TPP1 antibody (HA723972) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Raji cell lysate (20 µg/Lane) Lane 5: 786-0 cell lysate (20 µg/Lane) Lane 6: A431 cell lysate (20 µg/Lane) Lane 7: JAR cell lysate (20 µg/Lane) Lane 8: U-87 MG cell lysate (20 µg/Lane) Predicted band size: 61 kDa Observed band size: 48/61 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723972) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TPP1 on different lysates with Rabbit anti-TPP1 antibody (HA723972) at 1/5,000 dilution. Lane 1: Mouse heart tissue lysate (20 µg/Lane) Lane 2: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane) Lane 4: Rat heart tissue lysate (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Rat skeletal muscle tissue lysate (20 µg/Lane) Predicted band size: 61 kDa Observed band size: 48/61 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723972) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-TPP1 antibody (HA723972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - mouse Tpp1
- synonym:Cln2; LPIC; TPP-1; TPP-I
- description:tripeptidyl peptidase I
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Tpp1 antibody
- Tpp1 gene knockdown
- Tpp1 cDNA
- Tpp1 protein
- Tpp1 other
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