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- domestic rabbit monoclonal (JE60-60)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of TFE3 on different lysates with Rabbit anti-TFE3 antibody (HA721896) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: A-172 cell lysate (30 µg/Lane) Lane 6: NIH/3T3 cell lysate (30 µg/Lane) Lane 7: C6 cell lysate (30 µg/Lane) Lane 8: PC-12 cell lysate (30 µg/Lane) Predicted band size: 61 kDa Observed band size: 70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721896) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TFE3 antibody (HA721896) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721896) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-TFE3 antibody (HA721896) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721896) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JE60-60)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (1O9E9)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
citations: 12 - mouse monoclonal (G138-312)
reactivity: human, mouse
application: western blot, chromatin immunoprecipitation
citations: 1
gene information - mouse Tfe3
- synonym:F830016E06Rik; Tcfe3; Tfe-3; bHLHe33
- description:transcription factor E3
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Tfe3 antibody
- Tfe3 gene knockdown
- Tfe3 cDNA
- Tfe3 other
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