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Figure: ACRP30headless (mouse) does not bind to rhTNF-R1 and rhOX40:Fc in an ELISA assay.Method: A 96 well ELISA plate was coated O/N at RT with 100 µl of 1 µg/ml rhTNF-R1:Fc (Prod. No. ALX-522-013) and 1 µg/ml rhOX40:Fc (Prod. No. ALX-522-022) in PBS. 100 µl of rmsACRP30headless at 8 µg/ml were mixed with 100 µl 0.5% FCS in PBS in the first well and a series of 1:1 dilutions was prepared for the following wells. Binding of rmsACRP30headless to the receptor:Fc was detected after 1 hr incubation at 37°C using a goat anti-human HRP conjugated antibody (Jackson lab; 1:1’000 dilution, 1 hr incubation). O-phenylenediamine dihydrochloride (OPD) was used as a substrate, and absorbance was measured at 490 nm in an ELISA reader.
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gene information - mouse Tcf7l1
- synonym:Tcf-3; Tcf3; bHLHb21
- description:transcription factor 7 like 1 (T cell specific, HMG box)
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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