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- mouse monoclonal (H-6)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 1 - mouse monoclonal (H-6)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (H-6)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE34-80)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of TAX1BP1 on different lysates with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721648) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TAX1BP1 on different lysates with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-TAX1BP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721648) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - mouse Tax1bp1
- synonym:1200003J11Rik; 1700069J21Rik; AA930106; D6Ertd404e; D6Ertd772e; T6bp; TXBP151
- description:Tax1 (human T cell leukemia virus type I) binding protein 1
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Tax1bp1 antibody
- Tax1bp1 gene knockdown
- Tax1bp1 cDNA
- Tax1bp1 other
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