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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
Western Blot: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.
quantity: 0.1 mg
price: 549 USD
to the supplier - mouse monoclonal (A-10)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - mouse monoclonal (A-10)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - domestic rabbit monoclonal (JE40-02)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of TAF1 on different lysates with Rabbit anti-TAF1 antibody (HA722747) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Predicted band size: 215 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722747) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TAF1 antibody (HA722747) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722747) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation 
Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). CHIP was performed on fos promoters using 5 µg of TAF1 antibody from Rockland (P/n 600-401-995) and an RNA PolII antibody. Image with data provided courtesy of Shiraz Mujtaba, Ph.D., Dept. of Structural & Chemical Biology, Mount Sinai School of Medicine.
quantity: 100 µg
price:
to the supplier
gene information - mouse Taf1
- synonym:AU015687; B430306D02Rik; Ccg-1; Ccg1; KAT4; N-TAF1; TAFII250; Taf2a; p250
- description:TATA-box binding protein associated factor 1
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product type
- Taf1 antibody
- Taf1 gene knockdown
- Taf1 cDNA
- Taf1 other
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