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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 11
Western blot analysis of rat brain membranes: - 1. Anti-KCNK2 (TREK-1) Antibody (#APC-047), (1:200).2. Anti-KCNK2 (TREK-1) Antibody, preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).
Peptide DPKSAAQNSKPRLSFSTK(C) corresponding to residues 8-25 of human KCNK2(AccessionO95069). Intracellular N-terminus.
Expression of TREK-1 in rat hippocampus. - Immunohistochemical staining of rat brain sections using Anti-KCNK2 (TREK-1) Antibody (#APC-047). TREK-1 staining (red) in the hippocampus is detected in the stratum radiatum in the neuropil. Similar diffused staining is observed for GLAST. GFAP staining the astrocyte marker is also shown.Adapted from Zhou M. et al. (2009) J. Neurosci. 29 8551. with permission of the Society for Neuroscience.
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to the supplier - guinea-pigs polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1 
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1,2. Guinea pig Anti-KCNK2 (TREK-1) Antibody (#APC-047-GP), (1:800).3,4. Guinea pig Anti-KCNK2 (TREK-1) Antibody, preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).
Expression of KCNK2 (TREK-1) in rat midbrain - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-KCNK2 (TREK-1) Antibody (#APC-047-GP), (1:400), followed by goat-anti-guinea pig-Cy3 antibody. TREK-1 staining (red) appears in neurons in the rat lateral substantia nigra (arrows). Nuclei are stained with DAPI (blue).
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to the supplier - mouse monoclonal (F-6)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunocytochemistry knockout validation
citations: 4 - domestic rabbit polyclonal
reactivity: human, mouse, bovine
application: western blot, immunohistochemistry, immunocytochemistry
citations: 2 - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of KCNK2 on rat brain lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat adrenaline tissue using anti-KCNK2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-06, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KCNK2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-06, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
gene information - mouse TREK 1
- synonym:A430027H14Rik; AI848635; TREK-1
- description:potassium channel, subfamily K, member 2
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product type
- TREK 1 antibody
- TREK 1 gene knockdown
- TREK 1 cDNA
- TREK 1 other
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