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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Stanniocalcin 1 / STC on different lysates with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/5,000 dilution. Lane 1: SK-OV-3 cell lysate Lane 2: U-87 MG cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse small intestine tissue lysate Lane 5: Mouse pancreas tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat colon tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER61893) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of U-87 MG cells labeling Stanniocalcin 1 / STC with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER61893) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry 
Western blot analysis of extracts of mouse lung, using STC1 Antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using STC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using STC1 antibody at dilution of 1:100 (40x lens).
quantity: 0.05 mL
price:
to the supplier - mouse monoclonal (1A3)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section - domestic goat polyclonal
reactivity: human, mouse
application: western blot, neutralization
gene information - mouse Stc1
- synonym:Stc
- description:stanniocalcin 1
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