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- domestic rabbit polyclonal (NA)
reactivity: mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 2
Western blot analysis of rat brain lysate: - 1. Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003), (1:200). 2. Anti-Somatostatin Receptor Type 3 Antibody, preincubated with Somatostatin Receptor Type 3 Blocking Peptide (#BLP-SR003).
Expression of SSTR3 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003). A. SSTR3 appears in Purkinje cells (red, arrow points at an example). B. Staining of Purkinje nerve cells with mouse anti parvalbumin (a calcium binding protein, green). C. Confocal merge of SSTR3 and parvalbumin demonstrates the co-localization of these proteins in Purkinje cells (arrow points at a Purkinje cell pointed at in A and B).
Peptide KVRSTTRRVRAPSC corresponding to amino acid residues 233-246 of rat SSTR3 (AccessionP30936). Intracellular betweentransmembrane domains 5and 6.
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to the supplier - domestic rabbit monoclonal (JE63-87)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of SSTR3 on U87MG cell lysates with Rabbit anti-SSTR3 antibody (HA721024) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721024) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SSTR3 antibody (HA721024) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721024) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SSTR3 antibody (HA721024) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721024) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - mouse Sstr3
- synonym:Smstr-3; Smstr3; sst3
- description:somatostatin receptor 3
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- Sstr3 antibody
- Sstr3 gene knockdown
- Sstr3 cDNA
- Sstr3 other
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