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- mouse monoclonal (4H8)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of Sox-5 expression in HepG2 (A), SHSY5Y (B), mouse heart (C) whole cell lysates.
Immunohistochemical analysis of Sox-5 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Sox-5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.05 mL
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gene information - mouse Sox5
- synonym:A730017D01Rik; AI528773
- description:SRY (sex determining region Y)-box 5
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Sox5 antibody
- Sox5 gene knockdown
- Sox5 cDNA
- Sox5 other
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