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- domestic goat polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
citations: 8
SOX3 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).
quantity: 100 ug (also 25 ug)
price: 469 USD
to the supplier - mouse monoclonal (16-C2)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation - domestic rabbit polyclonal
reactivity: mouse
application: immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of SOX3 on different lysates with Rabbit anti-SOX3 antibody (HA500388) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: Human cerebellum tissue lysate (20 µg/Lane) Lane 5: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 6: Rat cerebellum tissue lysate (20 µg/Lane) Predicted band size: 45 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
gene information - mouse Sox3
- synonym:Sox-3
- description:SRY (sex determining region Y)-box 3
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Sox3 antibody
- Sox3 gene knockdown
- Sox3 cDNA
- Sox3 other
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