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- domestic rabbit monoclonal (6D3F6)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chicken, bovine
application: western blot, ELISA, immunohistochemistry - domestic rabbit monoclonal (JG36-86)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of SOCS2 on different lysates with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/1,000 dilution. Lane 1: SH-SY-5Y cell lysate (40 µg/Lane) Lane 2: NIH/3T3 cell lysate (40 µg/Lane) Lane 2: PC-12 cell lysate (40 µg/Lane) Lane 3: Mouse thymus tissue lysate (40 µg/Lane) Lane 4: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes 53 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-40) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-40) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of LOVO cells labeling SOCS2 with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
citations: 3
gene information - mouse Socs2
- synonym:8030460M17; AI527257; AW108012; CIS2; Cish2; D130043N08Rik; JAB; SOCS-2; SSI-2; hg
- description:suppressor of cytokine signaling 2
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product type
- Socs2 antibody
- Socs2 gene knockdown
- Socs2 cDNA
- Socs2 other
species
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