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- domestic rabbit polyclonal (A180)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - frozen section
citations: 3 - domestic rabbit monoclonal (JA11-57)
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of gamma Sarcoglycan on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-gamma Sarcoglycan antibody (ET1704-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
gene information - mouse Sgcg
- synonym:35kDa; 5430420E18Rik; AI642964; gamma-SG
- description:sarcoglycan, gamma (dystrophin-associated glycoprotein)
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