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- mouse monoclonal (3G4)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, proximity ligation assay
citations: 54 - domestic rabbit monoclonal (7Q8Q6)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - mouse monoclonal (56-Y)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 4 - domestic rabbit monoclonal (JB51-34)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Dcp1a on different lysates with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: MOLT-4 cell lysate Lane 3: HepG2 cell lysate Lane 4: HeLa cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-26) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Dcp1a antibody (ET7107-26) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-26) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (3G4)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 4
gene information - mouse SMIF
- synonym:1110066A22Rik; 4930568L04Rik; AU019772; D14Ertd817e; Mitc1; SMIF
- description:decapping mRNA 1A
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- SMIF antibody
- SMIF gene knockdown
- SMIF cDNA
- SMIF other
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