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- domestic rabbit polyclonal
reactivity: mouse
application: western blot, ELISA
citations: 1
Western blot using Rockland's Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0?ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal
reactivity: mouse
application: western blot - domestic rabbit polyclonal
reactivity: mouse
application: western blot, ELISA, immunoprecipitation
gene information - mouse Rif1
- synonym:5730435J01Rik; 6530403D07Rik; AU016181; AW549474; D2Ertd145e
- description:replication timing regulatory factor 1
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- Rif1 antibody
- Rif1 gene knockdown
- Rif1 cDNA
- Rif1 other
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