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- domestic rabbit monoclonal (PSH01-94)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (HA721752) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (30 µg/Lane) Lane 2: U-2 OS cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: HeLa cell lysate (30 µg/Lane) Lane 5: HepG2 cell lysate (30 µg/Lane) Lane 6: TT cell lysate (30 µg/Lane) Lane 7: Jurkat cell lysate (30 µg/Lane) Lane 8: NIH/3T3 cell lysate (30 µg/Lane) Lane 9: C2C12 cell lysate (30 µg/Lane) Lane 10: PC-12 cell lysate (30 µg/Lane) Lane 11: C6 cell lysate (30 µg/Lane) Lane 12: Mouse liver tissue lysate (30 µg/Lane) Lane 13: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 70 kDa Exposure time: 6 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Jurkat cells labeling FAM134B with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM134B antibody (HA721752) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH01-94)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of FAM134B on different lysates with Rabbit anti-FAM134B antibody (HA750768) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (30 µg/Lane) Lane 2: U-2 OS cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: HeLa cell lysate (30 µg/Lane) Lane 5: HepG2 cell lysate (30 µg/Lane) Lane 6: TT cell lysate (30 µg/Lane) Lane 7: Jurkat cell lysate (30 µg/Lane) Lane 8: NIH/3T3 cell lysate (30 µg/Lane) Lane 9: C2C12 cell lysate (30 µg/Lane) Lane 10: PC-12 cell lysate (30 µg/Lane) Lane 11: C6 cell lysate (30 µg/Lane) Lane 12: Mouse liver tissue lysate (30 µg/Lane) Lane 13: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 55 kDa Observed band size: 70 kDa Exposure time: 6 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Jurkat cells labeling FAM134B with Rabbit anti-FAM134B antibody (HA750768) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM134B antibody (HA750768) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section 
Western Blot: FAM134B Antibody [NBP1-59817] - 1: HEK293T 2: HEK293T mouse FAM134B over-expression, 1 ug 3: HEK293T mouse FAM134B over-expression, 4 ug 4: HEK293T human FAM134B over-expression, 1 ug 5: HEK293T human FAM134B over-expression, 4 ug. This image was submitted via customer Review.
quantity: 100 ul
price: 409 USD
to the supplier
gene information - mouse Retreg1
- synonym:1810015C04Rik; AU015349; Fam134b; Retreg1d
- description:reticulophagy regulator 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Retreg1 antibody
- Retreg1 gene knockdown
- Retreg1 cDNA
- Retreg1 other
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