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- domestic rabbit monoclonal (JE64-33)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of hnRNP G-T on different lysates with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/5,000 dilution. Lane 1: 293 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721104) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of hnRNP G-T on rat testis tissue lysates with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling hnRNP G-T with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta Ⅲ tubulin (M0805-8, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (RR-17)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation - domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation
gene information - mouse Rbmxl2
- synonym:1700012H05Rik; Hnrnpg-t
- description:RNA binding motif protein, X-linked-like 2
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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- Rbmxl2 antibody
- Rbmxl2 gene knockdown
- Rbmxl2 cDNA
- Rbmxl2 other
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