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- mouse monoclonal (E-6)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 1 - mouse monoclonal (E-6)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (E-6)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE35-61)
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of RAP1A + RAP1B on different lysates with Rabbit anti-RAP1A + RAP1B antibody (HA721335) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721335) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HEK-293 cells labeling RAP1A + RAP1B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721335, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (36E1)
reactivity: human, mouse, rat, bovine
application: western blot
citations: 3
gene information - mouse Rap1b
- synonym:2810443E11Rik
- description:RAS related protein 1b
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- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Rap1b antibody
- Rap1b gene knockdown
- Rap1b cDNA
- Rap1b other
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