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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of PSMB10 on different lysates with Rabbit anti-PSMB10 antibody (HA500393) at 1/500 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 26 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500393) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PSMB10 antibody (HA500393) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500393) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of SH-SY5Y cells labeling PSMB10 with Rabbit anti-PSMB10 antibody (HA500393) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMB10 antibody (HA500393) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (PSH01-58)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of PSMB10 on different lysates with Rabbit anti-PSMB10 antibody (HA721701) at 1/1,000 dilution. Lane 1: HeLa treated with 100ng/mL IFN-γ for 48 hours cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Daudi cell lysate (20 µg/Lane) Lane 4: Raji cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: Ramos cell lysate (20 µg/Lane) Lane 7: U-937 cell lysate (20 µg/Lane) Lane 8: HL-60 cell lysate (20 µg/Lane) Lane 9: Jurkat cell lysate (20 µg/Lane) Lane 10: SiHa cell lysate (20 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 25/29 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721701) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10 antibody (HA721701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH01-58)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of PSMB10 on different lysates with Rabbit anti-PSMB10 antibody (HA750734) at 1/1,000 dilution. Lane 1: HeLa treated with 100ng/mL IFN-γ for 48 hours cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Daudi cell lysate (20 µg/Lane) Lane 4: Raji cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: Ramos cell lysate (20 µg/Lane) Lane 7: U-937 cell lysate (20 µg/Lane) Lane 8: HL-60 cell lysate (20 µg/Lane) Lane 9: Jurkat cell lysate (20 µg/Lane) Lane 10: SiHa cell lysate (20 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 25/29 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750734) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PSMB10 antibody (HA750734) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750734) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - mouse Psmb10
- synonym:Mecl-1; Mecl1
- description:proteasome (prosome, macropain) subunit, beta type 10
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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- Psmb10 antibody
- Psmb10 gene knockdown
- Psmb10 cDNA
- Psmb10 other
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