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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, flow cytometry, enzyme immunoassay, immunohistochemistry - paraffin section
Overlay histogram showing A549 cells stainedwith MBS9207635 (green line). The cells were fixedwith 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9207635, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed(OH191631)at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
MBS9207635_IHC staining PRODH in H. skeletal musclesections by Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.
Anti-PRODH Antibody (Center) at 1: 2000 dilution + MDA-MB-468 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5%NFDM/TBST.
quantity: 0.08 mL
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to the supplier - domestic goat polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, enzyme immunoassay
gene information - mouse Prodh
- synonym:Pro-1; Pro1; Ym24d07
- description:proline dehydrogenase
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product type
- Prodh antibody
- Prodh gene knockdown
- Prodh cDNA
- Prodh other
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