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- mouse monoclonal (D-5)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - mouse monoclonal (D-5)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (D-5)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE64-38)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of TAPP-1 on different lysates with Rabbit anti-TAPP-1 antibody (HA722361) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: C6 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722361) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa cells labeling TAPP-1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722361, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
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gene information - mouse Plekha1
- synonym:AA960558; C920009D07Rik; TAPP1
- description:pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Plekha1 antibody
- Plekha1 gene knockdown
- Plekha1 cDNA
- Plekha1 other
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