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- domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - mouse monoclonal (OTI7H2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE64-05)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of PIN4 on different lysates with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Mouse stomach tissue lysate (40 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PIN4 on different lysates with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PIN4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721121) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HEK-293 cells labeling PIN4 with Rabbit anti-PIN4 antibody (HA721121) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PIN4 antibody (HA721121) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
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gene information - mouse Pin4
- synonym:2410002I22Rik; EPVH; Par14
- description:protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
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product type
- Pin4 antibody
- Pin4 gene knockdown
- Pin4 cDNA
- Pin4 other
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