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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 2

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite.
quantity: 100 µl
price:
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunohistochemistry, immunoprecipitation

Western Blot of rabbit anti-PDI alpha antibody. Lane 1: Rat Brain. Lane 2: Rat Heart. Lane 3: Rat Kidney. Lane 4: Rat Liver. Lane: 5 Rat Lung. Lane 6:Rat Pancreas. Lane 7: Rat Sketetal Muscle. Lane 8:Rat Spleen. Lane 9: Rat Testes. Lane 10: Rat Thymus. Lane11: C2C12. Lane 12: NIH 3T3 Ctrl. Lane 13: Pam212. Primary antibody: PID antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rabbit IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4°C. Predicted/Observed size: 56.9 kDa/58kD. Other band(s): none.
quantity: 100 µL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of PDI on different lysates using anti- PDI antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: PANC cell lysate Lane 4: HepG2 cell lysate Lane 5: HUVEC cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: L929 cell lysate Lane 8: F9 cell lysate Lane 9: Jurkat cell lysate Lane 10: A431 cell lysate Lane 11: A549 cell lysate Lane 12: Mouse liver tissue lysate Lane 13: Mouse kidney tissue lysate Lane 14: Mouse brain tissue lysate Lane 15: Human kidney tissue lysate Lane 16: Human brain tissue lysate Lane 17: Human liver tissue lysate
ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (JF97-08)
reactivity: human, mouse
application: western blot

Western blot analysis of PDIA2 on different lysates with Rabbit anti-PDIA2 antibody (ET1702-70) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Human liver tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: Lane 1: 1 minute 18 seconds; Lane 2-3: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - mouse Pdia2
- synonym:1810041F13Rik; AI661267; Pdip; Pdipl
- description:protein disulfide isomerase associated 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Pdia2 antibody
- Pdia2 gene knockdown
- Pdia2 cDNA
- Pdia2 protein
- Pdia2 other
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