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- mouse monoclonal (2B11)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Pannexin 2 on HepG2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-05, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PANX2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-05, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Pannexin 2 antibody (ER1901-05) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-05) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry 
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Pannexin 2 (extracellular) Antibody (#ACC-232), (1:200).3,4. Anti-Pannexin 2 (extracellular) Antibody, preincubated with Pannexin 2 (extracellular) Blocking Peptide (#BLP-CC232).
Peptide (C)EEPIY(S)YTPHNFTRD corresponding to amino acid residues 76-90 of human PANX2 (AccessionQ96RD6). 1stextracellular loop.
Expression of Pannexin 2 in human U-87 MG glioblastoma cell line - Cell surface detection ofPannexin 2 in live intact human U-87 MG glioblastoma cells. A. Cells were stained withAnti-Pannexin 2 (extracellular)Antibody (#ACC-232) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
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gene information - mouse Panx2
- description:pannexin 2
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product type
- Panx2 antibody
- Panx2 gene knockdown
- Panx2 cDNA
- Panx2 other
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