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- mouse monoclonal (E-4)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - mouse monoclonal (E-4)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (E-4)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE56-41)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of NUDT5 on different lysates with Rabbit anti-NUDT5 antibody (HA720015) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720015) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NUDT5 on different lysates with Rabbit anti-NUDT5 antibody (HA720015) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NUDT5 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720015) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NUDT5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720015, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293 cell lysate
quantity: 100μl
price: 385.00 USD
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gene information - mouse Nudt5
- description:nudix (nucleoside diphosphate linked moiety X)-type motif 5
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product type
- Nudt5 antibody
- Nudt5 gene knockdown
- Nudt5 cDNA
- Nudt5 other
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