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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 5
Western blot analysis of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (100 ng): - 1. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:200) 2. Anti-Neurotrophin 3 (NT-3) Antibody, preincubated with Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003).
Expression of Neurotrophin 3 in mouse spinal cord - Immunohistochemical staining of perfusion-fixed frozen mouse spinal cord sections with Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 (green). The same sections were stained with mouse anti-neurofilament 200 (NF) (red). Merge of the two images demonstrates the presence of NT-3 in gray matter which colocalizes with NF only in the soma of some neurons (arrow).
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis using Anti-proNT-3 Antibody (#ANT-012) (1:600): - 1. Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (200 ng).2. Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (200 ng).3. Native mouse NGF 2.5S protein (>95%) (#N-100), (200 ng).4. Recombinant human beta-NGF protein (#N-245), (200 ng).5. Recombinant human BDNF protein (#B-250), (200 ng).6. Recombinant mouse proNGF protein (#N-250), (200 ng).7. Recombinant mouse proBDNF protein (#B-240), (200 ng).8. Recombinant proNT-3, (10 ng).
Expression of proNT-3 in rat brain - Immunohistochemical staining of rat Islands of Calleja (IOC) using Anti-proNT-3 Antibody (#ANT-012). A. proNT-3 staining (green) overlaps neuronal nitric oxide synthase (nNOS red) while a cluster of cells covers a part of the island (DAPI blue counterstain) but not a cell-poor area (star). B. The distribution of proNT-3 is shown alone. Note that staining is more intense in the cell poor area demarcated by white line. C. The distribution of neuronal NOS is most intense in the cell-poor region.
Western blot analysisusing Anti-proNT-3 Antibody (#ANT-012) (1:600): - 1.Recombinant human Neurotrophin-3 (NT-3) protein(#N-260)(200 ng).2.Recombinant human Neurotrophin-4 (NT-4) protein(#N-270) (200 ng).3.Native mouse NGF 2.5S protein ( 95%)(#N-100) (200 ng).4.Recombinant human beta-NGF protein(#N-245) (200 ng).5.Recombinant human BDNF protein(#B-250)(200 ng).6.Recombinant mouse proNGF protein(#N-250)(200 ng).7.Recombinant mouse proBDNF protein(#B-240)(200 ng).8. Recombinant proNT-3 (10 ng).
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - mouse monoclonal (OTI5A2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - mouse monoclonal (OTI5A2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - mouse Ntf3
- synonym:AI316846; AI835689; HDNF; NGF-2; Nt3; Ntf-3
- description:neurotrophin 3
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