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- domestic rabbit monoclonal (SN07-11)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of NSD3 on different lysates with Rabbit anti-NSD3 antibody (ET1611-39) at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 162 kDa Observed band size: 162 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-39) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NSD3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-NSD3 antibody (ET1611-39) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-39) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (H-8)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - mouse monoclonal (E-3)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
gene information - mouse Nsd3
- synonym:6720429E03; A530023P05; AI528490; WHISTLE; Whsc1l1
- description:nuclear receptor binding SET domain protein 3
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product type
- Nsd3 antibody
- Nsd3 gene knockdown
- Nsd3 cDNA
- Nsd3 other
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