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- domestic rabbit monoclonal (JE30-94)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (HA721946) at 1/1,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: U-2 OS cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (30 µg/Lane) Lane 5: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 15 kDa Observed band size: 37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-BNP antibody (HA721946) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721946) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - Anti-BNP antibody [EPR22339-65] - BSA and Azide free
Abcam
catalog: ab243857
total number of products: 4domestic rabbit monoclonal
reactivity: mouse, rat
application: immunohistochemistry - paraffin section - Anti-NT-proBNP antibody [EPR25816-71] - BSA and Azide free (Capture)
Abcam
catalog: ab322929
total number of products: 4domestic rabbit monoclonal
reactivity: mouse
application: ELISA - Anti-NT-proBNP antibody [EPR25816-7] - BSA and Azide free (Detector)
Abcam
catalog: ab322930
total number of products: 4domestic rabbit monoclonal
reactivity: mouse
application: ELISA
gene information - mouse Nppb
- synonym:AA408272; BNF; BNP
- description:natriuretic peptide type B
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