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- domestic rabbit monoclonal (JE61-51)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of NOP10 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720060, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-NOP10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720060, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NOP10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720060, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (6H6)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
gene information - mouse Nop10
- synonym:1110036B12Rik; NOP10P; Nola3
- description:NOP10 ribonucleoprotein
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Nop10 antibody
- Nop10 gene knockdown
- Nop10 cDNA
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