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- domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot
Western blot analysis of NDNF on different lysates with Rabbit anti-NDNF antibody (HA500571) at 1/10,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 75 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500571) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of extracts of various cell lines, using NDNF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
quantity: 0.05 mL
price:
to the supplier
gene information - mouse Ndnf
- synonym:A930038C07Rik; epidermacan
- description:neuron-derived neurotrophic factor
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- Ndnf antibody
- Ndnf gene knockdown
- Ndnf other
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