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- domestic rabbit monoclonal (JE64-30)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody at 1/500 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 109 kDa Observed band size: 109 kDa Exposure time: 1 minute; 10 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody MYO19 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of NCI-H441 cells labeling MYO19 with Rabbit anti-MYO19 antibody (HA721051) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MYO19 antibody (HA721051) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
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gene information - mouse Myo19
- synonym:1110055A02Rik; Myohd1
- description:myosin XIX
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product type
- Myo19 antibody
- Myo19 cDNA
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