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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR27201-55] - BSA and Azide fre ...
Abcam
catalog: ab315225domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal (PSH15-17)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of MUL1 on different lysates with Rabbit anti-MUL1 antibody (HA723721) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: RD cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: Mouse thymus tissue lysate (low expression) (40 µg/Lane) Lane 8: Mouse kidney tissue lysate (40 µg/Lane) Lane 9: Rat thymus tissue lysate (low expression) (40 µg/Lane) Lane 10: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 37 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723721) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MUL1 antibody (HA723721) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723721) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH15-17)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of MUL1 on different lysates with Rabbit anti-MUL1 antibody (HA751546) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: RD cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: Mouse thymus tissue lysate (low expression) (40 µg/Lane) Lane 8: Mouse kidney tissue lysate (40 µg/Lane) Lane 9: Rat thymus tissue lysate (low expression) (40 µg/Lane) Lane 10: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 37 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751546) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MUL1 antibody (HA751546) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751546) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - mouse Mul1
- synonym:0610009K11Rik; AV000801; Gide
- description:mitochondrial ubiquitin ligase activator of NFKB 1
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Mul1 antibody
- Mul1 gene knockdown
- Mul1 cDNA
- Mul1 other
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