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- mouse monoclonal (SM3)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - mouse monoclonal (SM3)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (SM3)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (SN06-80)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of MUC1 on different lysates with Rabbit anti-MUC1 antibody (ET1611-14) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (negative) (20 µg/Lane) Lane 3: T-47D cell lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 122 kDa Observed band size: 17~24 kDa Exposure time: 3 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-14) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa (positive) and HCT 116 (negative) labeling MUC1 with Rabbit anti-MUC1 antibody (ET1611-14) at 1/500 dilution and competitor's antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MUC1 antibody (ET1611-14) at 1/500 dilution and competitor's antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Western blot analysis of MUC1 on different lysates with Rabbit anti-MUC1 antibody (ET1611-14) at 1/2,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si MUC1#1 cell lysate Lane 3: Hela-si MUC1#2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 122 kDa Observed band size: 17~24 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. ET1611-14 was shown to specifically react with MUC1 in Hela-si NT cells. Weakened bands were observed when Hela-si MUC1 sample were tested. Hela-si NT and Hela-si MUC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1611-14, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier - domestic rabbit monoclonal (SN06-80)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of MUC1 on different lysates with Rabbit anti-MUC1 antibody (HA750245) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (negative) (20 µg/Lane) Lane 3: T-47D cell lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 122 kDa Observed band size: 17~24 kDa Exposure time: 3 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750245) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa (positive) and HCT 116 (negative) labeling MUC1 with Rabbit anti-MUC1 antibody (HA750245) at 1/500 dilution and competitor's antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MUC1 antibody (HA750245) at 1/500 dilution and competitor's antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Western blot analysis of MUC1 on different lysates with Rabbit anti-MUC1 antibody (HA750245) at 1/2,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si MUC1#1 cell lysate Lane 3: Hela-si MUC1#2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 122 kDa Observed band size: 17~24 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. ET1611-14 was shown to specifically react with MUC1 in Hela-si NT cells. Weakened bands were observed when Hela-si MUC1 sample were tested. Hela-si NT and Hela-si MUC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1611-14, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - mouse Muc1
- synonym:CD227; EMA; Muc-1
- description:mucin 1, transmembrane
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- Muc1 antibody
- Muc1 gene knockdown
- Muc1 cDNA
- Muc1 protein
- Muc1 other
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