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- mouse monoclonal (OTI1C5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1 - mouse monoclonal (OTI1C5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Western Blot: KIF2C Antibody (OTI1C5) - Azide and BSA Free [NBP2-71667] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF2C.
quantity: 100 ug
price: 579 USD
to the supplier - domestic rabbit monoclonal (PSH01-51)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of MCAK on different lysates with Rabbit anti-MCAK antibody (HA721694) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721694) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MCAK on different lysates with Rabbit anti-MCAK antibody (HA721694) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MCAK KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721694) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MCAK antibody (HA721694) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721694) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH01-51)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of MCAK on different lysates with Rabbit anti-MCAK antibody (HA750727) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750727) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MCAK on different lysates with Rabbit anti-MCAK antibody (HA750727) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MCAK KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750727) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MCAK antibody (HA750727) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750727) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
gene information - mouse MCAK
- synonym:4930402F02Rik; ESTM5; Knsl6; MCAK; X83316
- description:kinesin family member 2C
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- MCAK antibody
- MCAK gene knockdown
- MCAK cDNA
- MCAK other
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