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- domestic rabbit monoclonal (JE47-60)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Lysophospholipase 1 on different lysates with Rabbit anti-Lysophospholipase 1 antibody (ET7109-63) at 1/500 dilution. Lane 1: Mouse stomach tissue lysate (20 µg/Lane) Lane 2: Daudi cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 25/30 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-63) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ICC staining of Lysophospholipase 1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Lysophospholipase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-63, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] - BSA and Azide free
Abcam
catalog: ab247525
total number of products: 4domestic rabbit monoclonal
reactivity: human, mouse
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot
gene information - mouse Lypla1
- synonym:APT-1; Gm39587; LPL-I; Pla1a
- description:lysophospholipase 1
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product type
- Lypla1 antibody
- Lypla1 gene knockdown
- Lypla1 cDNA
- Lypla1 protein
- Lypla1 other
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