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- mouse monoclonal (203/14F11)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 3
Detection of Leukotriene B4 R1 antibody in Human Blood Monocytes antibody by Flow Cytometry.
quantity: 100 Tests
price: 379 USD
to the supplier - domestic rabbit monoclonal (JE58-77)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Leukotriene B4 Receptor on different lysates with Rabbit anti-Leukotriene B4 Receptor antibody (HA721994) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721994) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Leukotriene B4 Receptor on different lysates with Rabbit anti-Leukotriene B4 Receptor antibody (HA721994) at 1/1,000 dilution. Lane 1: Mouse skin tissue lysate (hot lysis) Lane 2: Mouse lung tissue lysate (no heat) Lane 3: Rat lung tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721994) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Leukotriene B4 Receptor antibody (HA721994) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721994) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (014)
reactivity: human, mouse
application: ELISA
gene information - mouse Ltb4r1
- synonym:BLT1; BLTR; Ltb4r; mBLTR
- description:leukotriene B4 receptor 1
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product type
- Ltb4r1 antibody
- Ltb4r1 gene knockdown
- Ltb4r1 cDNA
- Ltb4r1 other
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