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- mouse monoclonal (D-6)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 2 - mouse monoclonal (G-6)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1 - mouse monoclonal (D-6)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of LSS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500011, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: K562 cell lysate Lane 3: Human liver tissue lysate
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-LSS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500011, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-LSS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500011, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - mouse Lss
- synonym:2810025N20Rik; D10Ertd116e; Osc
- description:lanosterol synthase
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product type
- Lss antibody
- Lss gene knockdown
- Lss cDNA
- Lss other
species
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