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- mouse monoclonal (TT-07)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
citations: 1 - domestic rabbit monoclonal (JE66-62)
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of LFNG on different lysates with Rabbit anti-LFNG antibody (HA721367) at 1/1,000 dilution. Lane 1: Hela cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: HL-60 cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: HT-29 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: PC-12 cell lysate (15 µg/Lane) Lane 8: Mouse testis tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HUVEC cells labeling LFNG with Rabbit anti-LFNG antibody (HA721367) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LFNG antibody (HA721367) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunocytochemistry analysis of MCF7 cells labeling LFNG with Rabbit anti-LFNG antibody (HA721367) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LFNG antibody (HA721367) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
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gene information - mouse Lfng
- synonym:AW061165
- description:LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
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product type
- Lfng antibody
- Lfng gene knockdown
- Lfng cDNA
- Lfng other
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