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- domestic rabbit polyclonal (NA)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry
citations: 3
Western blot analysis of mouse brain lysate: - 1. Anti-KChIP2 Antibody (#APC-142), (1:200).2. Anti-KChIP2 Antibody, preincubated with KChIP2 Blocking Peptide (#BLP-PC142).
Expression of KChIP2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-KChIP2 Antibody (#APC-142), (1:400), followed by goat-anti-rabbit-Alexa-488 (green). KChIP2 staining in the hippocampal CA1 region appears in neuronal profiles (arrows) in the pyramidal layer (Pyr). DAPI is used to stain nuclei (blue).
Peptide (C)RGQGRKESLSDSRD corresponding to amino acid residues 2-15 of human KChIP2 (AccessionQ9NS61). Cytoplasmic N-terminus.
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to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of KCNIP2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500491, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat brain tissue lysate
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KCNIP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500491, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-KCNIP2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500491, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - mouse monoclonal (9K9)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - mouse monoclonal
reactivity: mouse
application: immunohistochemistry - paraffin section - mouse monoclonal
reactivity: mouse
application: immunohistochemistry - paraffin section
gene information - mouse Kcnip2
- synonym:KChIP2
- description:Kv channel-interacting protein 2
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- Kcnip2 antibody
- Kcnip2 gene knockdown
- Kcnip2 cDNA
- Kcnip2 other
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