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- mouse monoclonal (HOX5I043)
reactivity: human, mouse
application: western blot, immunoprecipitation
citations: 2 - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of HOXA9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500077, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Mouse kidney tissue lysate
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HOXA9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500077, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - mouse Hoxa9
- synonym:D6a9; Hox-1.7
- description:homeobox A9
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product type
- Hoxa9 antibody
- Hoxa9 gene knockdown
- Hoxa9 cDNA
- Hoxa9 other
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