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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
Western blot analysis of HERC6 on different lysates with Rabbit anti-HERC6 antibody (HA500594) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: HL-60 cell lysate Lane 3: HeLa cell lysate Lane 4: BxPC-3 cell lysate Lane 5: SK-OV-3 cell lysate Lane 6: Rat colon tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 115 kDa Observed band size: 100 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500594) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of HERC6 on different lysates with Rabbit anti-HERC6 antibody (HA500594) at 1/5,000 dilution. Lane 1: Mouse colon tissue lysate Lane 2: Mouse embryo tissue lysate Lane 3: Rat embryo tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 115 kDa Observed band size: 100 kDa Exposure time: Lane 1: 14 seconds; Lane 2-3: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500594) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
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gene information - mouse Herc6
- synonym:1700121D12Rik; 2510038N07Rik; 4930427L17Rik; AI451296; CEB1; Herc5
- description:hect domain and RLD 6
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product type
- Herc6 antibody
- Herc6 gene knockdown
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