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- domestic rabbit monoclonal (SR38-03)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated CRC whole cell lysates Lane 2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates
ICC staining of Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JU43-48)
reactivity: human, mouse, rat
application: western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section 
Western blot analysis of Histone H1.2 on different lysates with Rabbit anti-Histone H1.2 antibody (ET1706-26) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Histone H1.2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 30 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-26) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H1.2 on different lysates with Rabbit anti-Histone H1.2 antibody (ET1706-26) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-26) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H1.2 on different lysates with Rabbit anti-Histone H1.2 antibody (ET1706-26) at 1/500 dilution. Lane 1: rat liver tissue lysate Lane 2: mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-26) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - mouse H1f4
- synonym:H1-4; H1.4; H1e; H1s-4; H1var2; Hist1h1e
- description:H1.4 linker histone, cluster member
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- H1f4 antibody
- H1f4 gene knockdown
- H1f4 cDNA
- H1f4 other
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