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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay
MBS9604283 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Western blot analysis of GPR84 expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.
quantity: 0.1 mL
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6): - 1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).
Expression of GPR84 in mouse fornix in a kainic acid-induced model of temporal lobe epilepsy - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. In untreated mouse sections GPR84 immunoreactivity (green) is detected in astrocyte profiles (arrows). B. GPR84 staining (green) in mouse sections obtained four days post kainate-induced seizures, shows an increase in number and size of astrocyte profiles stained for GPR84 (arrows). Cell nuclei are stained with DAPI (blue).
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: FITC
application: flow cytometry
Cell surface detection of GPR84 in live intact mouse BV-2 microglia cells: Cell surface detection of GPR84 in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-GPR84 (extracellular)-FITC Antibody (#AGR-052-F), (5 µg).
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to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
conjugate: PE
application: flow cytometry
Cell surface detection of GPR84 by direct flow cytometry in live intact mouse BV-2 microglia cells: Cell surface detection of GPR84 by direct flow cytometry in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-PE. Green line: Cells + Anti-GPR84 (extracellular)-PE Antibody (#AGR-052-PE), (2.5µg).
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gene information - mouse Gpr84
- synonym:EX33
- description:G protein-coupled receptor 84
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product type
- Gpr84 antibody
- Gpr84 gene knockdown
- Gpr84 cDNA
- Gpr84 other
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