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- domestic rabbit monoclonal (012)
reactivity: mouse
application: ELISA - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Glypican 4 on different lysates with Rabbit anti-Glypican 4 antibody (HA500432) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate (40 µg/Lane) Lane 2: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500432) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Glypican 4 with Rabbit anti-Glypican 4 antibody (HA500432) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Glypican 4 antibody (HA500432) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
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gene information - mouse Gpc4
- synonym:9530073D23Rik; AF311610; AI385680; AI661372; K-glypican
- description:glypican 4
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- Gpc4 antibody
- Gpc4 gene knockdown
- Gpc4 cDNA
- Gpc4 protein
- Gpc4 other
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