mouse Gm5593 antibody | 13 products in Validated Antibody Database; 17 cited in the literature; 41 total from 13 suppliers

Your Filters

product

cyclin B1 (GNS1) Antibody
Santa Cruz Biotechnology
catalog: sc-245

total number of products: 10

mouse monoclonal (GNS1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 244

quantity: 200 µg/ml
price: 279 USD
to the supplier
cyclin B1 (D-11) Antibody
Santa Cruz Biotechnology
catalog: sc-7393

total number of products: 10

mouse monoclonal (D-11)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 22

quantity: 200 µg/ml
price: 279 USD
to the supplier
cyclin B1 (GNS1) AC
Santa Cruz Biotechnology
catalog: sc-245 AC

total number of products: 10

mouse monoclonal (GNS1)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
citations: 15

quantity: 500 µg/ml, 25% ag
price: 366 USD
to the supplier
Cyclin B1
Proteintech Group
catalog: 55004-1-AP

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
citations: 86

The Cyclin B1 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human Cyclin B1. This antibody recognizes human antigen. The Cyclin B1 antibody has been validated for the following applications: ELISA, WB, IP, IF, IHC analysis.
quantity: 150 ul
price: 299 USD
to the supplier
Cyclin B1 Monoclonal Antibody (GNS1)
Invitrogen
catalog: MA5-14319

total number of products: 13

mouse monoclonal (GNS1)
reactivity: Chinese hamster, human, mouse, cow
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 23

quantity: 500µL
price: US 390
to the supplier
Cyclin B1 Monoclonal Antibody (V152)
Invitrogen
catalog: MA5-13128

total number of products: 13

mouse monoclonal (V152)
reactivity: Chinese hamster, human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15

quantity: 500µL
price: US 345
to the supplier
Cyclin B1 Monoclonal Antibody (GNS11)
Invitrogen
catalog: MA5-14327

total number of products: 13

mouse monoclonal (GNS11)
reactivity: Chinese hamster, human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 10

Immunofluorescent analysis of Cyclin B1 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Cyclin B1 monoclonal antibody (Product # MA5-14327) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human tonsil tissue. To expose target proteins, heat-induced antigen retrieval was performed by boiling tissue sections in 10mM sodium citrate buffer (pH 6) for 10-20 minutes. Tissues were probed with a Cyclin B1 monoclonal antibody (Product # MA5-14327) at a concentration of 3ug/ml. Detection was performed using an anti-mouse IgG-HRP secondary antibody followed by a colorimetric substrate

Immunoprecipitation of Cyclin B1 was performed on the Human Colon Carcinoma cell line LS174T. Antigen-antibody complexes were formed by incubating 500ul of LS174T whole cell lysate with 5ul of a Cyclin B1 monoclonal antibody (Product # MA5-14327). The immune complexes were captured using Protein A agarose, washed extensively, and eluted. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a membrane, and probed with a series of reagents to detect Cyclin B1
quantity: 500µL
price: US 345
to the supplier
Anti-Cyclin B1 antibody [EPR17060]
Abcam
catalog: ab181593

rabbit monoclonal (EPR17060)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 6

quantity: 10 µl, 100 µl
price:
to the supplier
Anti-Cyclin B1 (phospho S126) antibody
Abcam
catalog: ab55184

rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 µg
price:
to the supplier
Cyclin B1 antibody
GeneTex
catalog: GTX100911

rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
citations: 5

Sample (50 ug of HeLa whole cell lysate) GTX100911 diluted at 1:5000

Western blot analysis of cyclin B1 (GTX100911, upper panel) and beta-actin (GTX109639, lower panel) Sample (30 ug of whole cell lysate) A: U2OS B: U2OS treated 100ng/ml Nocodazole 16hr 10% SDS PAGE GTX100911 diluted at 1:500

Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. Cyclin B1 antibody (GTX100911) diluted at 1:500.
quantity: 100.00μl
price: 299 USD
to the supplier
Cyclin B1 Antibody
R&D Systems
catalog: AF6000

goat polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry
citations: 2

Detection of Human Cyclin B1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.2 ╡g/mL of Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band was detected for Cyclin B1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 369 USD
to the supplier
CCNB1 / Cyclin B1 Antibody (aa103-130) LS-C203981
LifeSpan Biosciences
catalog: LS-C203981

rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse CCNB1 Antibody (Center S123/S125)western blot of mouse NIH/3T3 cell line and mouse spleen tissue lysates (35 ug/lane). The mouse CCNB1 antibody detected the mouse CCNB1 protein (arrow).
quantity:
price:
to the supplier
Ccnb1 monoclonal antibody, clone V143.1
Abnova
catalog: MAB7000

total number of products: 6

mouse monoclonal (V143.1)
reactivity: mouse
application: western blot

quantity: 100 ug
price:
to the supplier
CCNB1 polyclonal antibody
Abnova
catalog: PAB0818

total number of products: 6

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - frozen section

quantity: 200 ug
price:
to the supplier
CCNB1 polyclonal antibody
Abnova
catalog: PAB18121

total number of products: 6

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 ug
price:
to the supplier
Cyclin B1 Antibody
Rockland Immunochemicals
catalog: 100-401-152

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation

Rockland's anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
quantity: 100 µL
price: 369 USD
to the supplier
Cyclin B1 phospho S126 Antibody
Rockland Immunochemicals
catalog: 600-401-464

rabbit polyclonal
reactivity: human, mouse, rat, dog, chimpanzee
application: western blot, ELISA, immunoprecipitation

Western blot using Rockland's Affinity Purified anti-Cyclin B 1 pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B 1 (arrowheads) in various whole cell lysates. Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 µg of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel. Transfer occurred overnight at 4° C at 15 mAmps. The membrane was blocked with 5% non-fat dry milk in TTBS for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature. After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (611-103-122) was added for 1 h at room temperature. After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min. Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results. Personnel Communication, Luca Cote, Temple U.
quantity: 100 µg
price: 369 USD
to the supplier
cyclin B1 (V152) Antibody
MyBioSource
catalog: MBS440095

mouse monoclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS, immunohistochemistry - paraffin section

quantity: 0.1 mg
price: 340 USD
to the supplier
Cyclin B1 Antibody (CCNB1 / 1098)
Novus Biologicals
catalog: NBP2-44687-0.1mg

mouse monoclonal (CCNB1/1098)
reactivity: human, mouse
application: immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Testicular Carcinoma stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)
quantity: 0.1 mg (also 0.02 mg)
price: 299 USD
to the supplier
Anti-Cyclin B1 Rabbit Monoclonal Antibody
Boster
catalog: M00745-2

rabbit monoclonal (EOG-3)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody(M00745-2). CCNB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCNB1 Antibody (M00745-2)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Cyclin B1 expression in HeLa cell lysate (M00745-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 monoclonal antibody (Catalog # M00745-2) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1
quantity: 100 ug/vial
price: 299 USD
to the supplier
Anti-Cyclin B1 antibody produced in rabbit
MilliporeSigma
catalog: SAB4503501

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 2

quantity:
price:
to the supplier