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- domestic rabbit monoclonal (JJ084-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JJ084-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Ubiquitin D on different lysates with Rabbit anti-Ubiquitin D antibody (ET1701-9) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 20 kDa Exposure time: 26 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-9) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ICC staining of Ubiquitin D in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Ubiquitin D in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section 
FAT10 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the hFAT10 antibody detected the hFAT10 protein (arrow).
FAT10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FAT10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of FAT10 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
quantity: 0.08 mL
price:
to the supplier
gene information - mouse FAT10
- synonym:FAT10
- description:ubiquitin D
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- FAT10 antibody
- FAT10 gene knockdown
- FAT10 cDNA
- FAT10 other
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