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- domestic rabbit polyclonal (1A3H4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic goat polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1 - domestic rabbit monoclonal (JE57-03)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Erlin-2 on different lysates with Rabbit anti-Erlin-2 antibody (HA720009) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720009) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Erlin-2 on different lysates with Rabbit anti-Erlin-2 antibody (HA720009) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Erlin-2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720009) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720009, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation
gene information - mouse Erlin2
- synonym:BC036333; C87251; Spfh2
- description:ER lipid raft associated 2
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Erlin2 antibody
- Erlin2 gene knockdown
- Erlin2 cDNA
- Erlin2 other
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